Hygromycin B Solution (50 mg/ml in PBS Buffer)

Produkt przeznaczony wyłącznie do badań. Nie nadaje się do spożycia. Więcej informacji na stronie producenta www.toku-e.com

CAS Number: 31282-04-9
Molecular Formula: C20H37N3O13
Molecular Weight: 527.52 g/mol
Form: Solution
Appearance: Clear and colorless or faint yellow solution
Solubility: Solution
Source: Biosynthetic: produced by Streptomyces hygroscopicus.
Melting Point: 160-180°C
Storage Conditions: 28°C.

Description
Hygromycin B is a unique aminoglycoside antibiotic derived from Streptomyces hygroscopicus. Hygromycin B solution is routinely used as a selective agent in cell culture or microbiology applications to isolate hygromycin B resistant cells.
This product is packaged as a solution at a concentration of 50 mg/mL in PBS buffer.

Mechanism
Hygromycin B inhibits protein synthesis by strengthening the interaction of tRNA binding in the ribosomal A-site. Hygromycin B also prevents mRNA and tRNA translocation by an unknown mechanism. These are unique mechanisms for an aminoglycoside antibiotic and they differ from the mode of action
neomycin, gentamicin, and G418.

Spectrum
Hygromycin B is effective against eukaryotic and prokaryotic cells.

Microbiology Applications
Hygromycin B can be used as a selection agent to isolate hygromycin b resistant bacteria and fungi.

Mammalian Biology Applications
Hygromycin B is routinely used as a selective agent in mammalian cell culture to isolate hygromycin B resistant cells after transfection. Selectable markers for hygromycin B resistant cells include the hyg or hph resistance genes which express a phosphotransferase that inactivates hygromycin B by phosphorylation. Effective working concentrations range from 100 – 1000 μg/mL.
For additional information regarding relevant cell lines, resistance plasmids, and culture media, please visit our cell culture database. Hygromycin B, 50mg/ml in PBS Buffer

References
Dai S., Zheng P., Marmey P., Zhang S., Tian W., Chen S., Beachy R.N. and Fauquet C. Comparative analysis of transgenic rice plants obtained by Agrobacterium-mediated transformation and particle bombardment. Molecular Breeding 7: 25–33, 2001. © 2001 Kluwer Academic Publishers.
Schindler, D. "Studies on the Mode of Action of Hygromycin B, an Inhibitor of Translocation in Eukaryotes." Nucleic Acids and Protein Synthesis 521.2 (1978): 459-69. www.ncbi.gov. Web. 6 Sept. 2012.