TRITICUM VULGARIS AGGLUTININ(Wheat Germ Agglutinin)

Applications
Lectins are very valuable in a wide variety of applications in vitro, including: blood grouping and erythrocyte polyagglutination studies, Mitogenic stimulation of lymphocytes, Lymphocyte subpopulation studies, Fractionation of cells and other particles, Histochemical studies of normal and pathological conditions.

Product Description
Lectins are proteins or glycoproteins of non-immune origin that agglutinate cells and/or precipitate complex carbohydrates. Lectins are capable of binding glycoproteins even in presence of various detergents. The agglutination activity of these highly specific carbohydrate-binding molecules is usually inhibited by a simple monosaccharide, but for some lectins, di, tri, and even polysaccharides are required. It is purified Triticum vulgrislectin (WGA) from Wheat Germ.

Biochem/physiol Actions
WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.

Typical Working Concentration
Reconstitute with Buffer to a concentration of 1 mg/ml.

Extinction Coefficient (E1%): 1.09 x 105 (Lit.)
Format: Lyophilized Powder
NOTES: Inhibitory Carbohydrate: GlcNAcb(1,4)GlcNAcb(1,4)GlcNAc>GlcNAcb(1,4)GlcNAc>Glc sialic acid(Neu5Ac)>>GalNAc.; Buffer: 0.01 M Phosphate - 0.15M NaCl, pH 7.2 - 7.4.; Stability: The lyophilized material is stable for several years when stored frozen. After reconstitution the material is stable for at least 1 year when stored frozen in aliquots with 0.1% sodium azide added as a preservative.
Specificity: Carbohydrate Specificity: (GlcNAc-b-(1,4)-GlcNAc)1-4> b-GleNAc>Neu5Ac
Solubility: Dissolvable in distilled water and 0.9% NaCl within 5 minutes.
Storage & Handling: -20 °C