BRCA Somatic Multiplex I (FFPE)

Format: FFPE

The BRCA Somatic Multiplex 1 Reference Standard in FFPE format is a highly-characterized, biologically-relevant quality control material used to assess the performance of NGS assays that detect somatic mutations. With this product you are able to: • Evaluate workflow integrity from pre-analytical DNA extraction to post-analytical bioinformatics • Analyze sensitivity and specificity of your assay • Optimize and validate new cancer panels and routinely monitor the performance of your assay.

Next generation sequencing (NGS) platforms give researchers much greater power when profiling tumor samples. However, the use of NGS presents challenges surrounding sample variability, platform bias, and potential failure to detect biomarkers. Horizon’s standards are appropriate for any NGS library preparation including whole-genome, whole-exome, custom capture and targeted amplicon panels. Horizon has developed this Quality-Seq (Q-Seq) NGS Reference Standard range to support the development and continued validation of Next Generation Sequencing platforms. The BRCA Somatic Multiplex 1 portfolio covers multiple endogenous SNPs, including those in difficult to sequence homopolymer regions.

The BRCA Somatic Multiplex 1 Reference Standard includes 16 mutations at 7.5% - 100% allelic frequency in genomic DNA and FFPE format. The multiple formats available allow you to validate both the pre-analytical and analytical portion of your workflow using an isogenic reference standard. The FFPE format BRCA Somatic Multiplex 1 reference standard allows you to evaluate and optimize DNA extraction protocols without wasting precious patient samples. In addition, the robustness of your NGS workflow may be tested to ensure that your library protocol and informatics pipeline can accommodate degraded FFPE samples. The BRCA Somatic Multiplex 1 reference standard provides a large range of allelic frequencies in 16 onco-relevant mutantations for use across multiple BRCA NGS gene panels.

Technical Data

Genes Covered: BRCA1, BRCA2, BRIP1, BARD1, NBN

Cosmics: 16

Fixation Method: 4% Formalin

Section Size:  15µm or 20µm

Cell Density: 3 x 10⁸ cells per block. Approx. 3.5 x 10⁵ cells per section

Expected DNA Yield: ≥ 400 ng using Promega Maxwell LEV Plus Extraction kit




Unit Size: 1 FFPE Section

Extractable DNA: ≥ 400 ng per section using Promega Maxwell LEV Plus FFPE DNA Extraction kit

Intended Use: For routine performance monitoring (Research Use Only)

General Information

Allelic Frequency: 7.5%-100%

Storage: 4˚C

Expiry: 48 months from the date of manufacture

Quality Control

Genotype: Sanger sequencing of locus specific PCR
Quality: Agarose gel electrophoresis
Quantification: Quantifluor™