Yeast DAmP Library for 842 essential genes - haploid

The Decreased Abundance by mRNA Perturbation (DAmP) Yeast Library is a  collection of Saccharomyces cerevisiae strains containing compromised  alleles of essential genes that exhibit modest growth defects. These  alleles are hypomorphic and cover > 950 of the essential yeast genes  as diploid and > 800 genes as haploid, enabling quantitative genetic  interaction measurements that allow in-depth functional dissection of  pathways and complexes.

The strategy for this library is based on  disruption of the 3’ UTR (untranslated region) with an antibiotic  resistance cassette (kanamycin), which destabilizes the corresponding  transcript and can reduce mRNA levels 4- to 10-fold. The library is  available in either haploid or heterozygous diploid formats.

Highlights

• Use for exploring interactions of essential genes
• Sensitive detection of growth rate changes
• High resolution functional analysis of the yeast genome
• Hypermorphic alleles cover > 950 of the essential yeast genes as diploid and > 800 genes as haploid

Note
The  creators of this resource list the number of genes they believe to be  essential in each collection in the product description. DAmP strains  were created for additional genes and are included in the collections.  The total the number of strains included in each collection is greater  than the number listed in the product description.

We provide  certain clone resources developed by leading academic laboratories. Many  of these resources address the needs of specialized research  communities not served by other commercial entities. In order to provide  these as a public resource, we depend on the contributing academic  laboratories for quality control.

Therefore, these are  distributed in the format provided by the contributing institution "as  is" with no additional product validation or guarantee. We are not  responsible for any errors or performance issues. Additional information  can be found in the product manual as well as in associated published  articles (if available). Alternatively, the source academic institution  can be contacted directly for troubleshooting.

We will ship your clone at room temperature via express delivery.  Store the stock clone at –80°C. Clones are provided as a live culture in  a 2 mL tube. Each tube contains:

•     Haploid DAmP strain: YEPD + G418 (200 ug/mL) + 15% glycerol
•     SD-URA + G418 (200 ug/mL) + 15% glycerol