
6X Loading Dye and SDS Solution
Opis
Thermo Scientific 6X DNA Loading Dye & SDS Solution is recommended for agarose gel analysis of DNA samples that contain high amounts of DNA binding proteins. The presence of SDS eliminates DNA-protein interactions and prevents gel-shifts. The high concentration of EDTA protects DNA from degradation by metal-dependent nucleases.
Highlights
- 1% SDS eliminates DNA-protein interactions, prevents appearance of additional bands due to annealing of DNA molecules with cohesive ends
- 100 mM EDTA inhibits metal-dependent nucleases
Applications
- Analysis of DNA samples with high amounts of DNA binding proteins
- Kinetic experiments
- DNA agarose gel analysis after DNA restriction digestions, ligation, or dephosphorylation reactions
Dane techniczne
Opakowanie | 5 x 1 ml |