
Agarase
Opis
Agarase specifically digests the agarose polysaccharide core into neoagaro-oligosaccharides (1). Agarase allows for gentle yet efficient recovery of DNA or RNA fragments from low melting point agarose. The recovered nucleic acids can be directly used for amplification, cloning, sequencing, etc.
Highlights
- Gentle – recovers intact DNA and RNA
- High recovery yields – > 90% of the starting DNA recovered following extraction
- Ideal for both long (> 30 kb) and short (< 100 bp) DNA fragments
- Active in TAE and TBE electrophoresis buffers
Applications
Quantitative recovery of DNA or RNA from low melting point (LM) agarose gels.
Note
- Incubate at 42°C.
- Conventional agarose is not suitable for digestion by Agarase.
- Activity of agarase in different buffers (in comparison to activity in assay buffer (1x TBE)):
1x TBE (90 mM Tris-borate, 2 mM EDTA, pH 8.3) - 100%.
1x TAE (40 mM Tris-acetate, 1 mM EDTA, pH 8.5) - 120%.
1x TPE (90 mM Tris-phosphate, 2 mM EDTA, pH 7.7) - 120%.
1x Bis-Tris (50 mM Bis-Tris-HCl, 1 mM EDTA, pH 6.5) - 150%
Dane techniczne
Opakowanie | 100 U |