Description: T4 ssRNA Ligase catalyses the ATP-dependent formation of phosphodiester bonds between 5’-phosphate and 3’-hydroxyl termini of oligonucleotides, single-stranded RNA and DNA. Note that the minimal substrate for T4 ssRNA Ligase is a nucleoside 3’,5’-biphosphate in intermolecular reaction and oligonucleotide of 8 bases in intramolecular reaction. The enzyme may be used for the ligation of ssRNA and DNA, 3´-termini of RNA labelling, synthesis of single-stranded oligoribo- and oligodeoxyribo-nucleotides and specific modifications of tRNAs.
Features:
– Ligates ssRNA or ssDNA fragments containing 5’-phosphate and 3’-hydroxyl termini
– ATP-dependent RNA Ligase
Applications:
– Ligation of ssRNA and DNA
– 3´-end labelling of single strand RNA
– Synthesis of single-stranded oligoribonucleotides and oligodeoxyribonucleotides
– Specific modifications of tRNAs
Specifications:
– Enzyme concentration: 10 U/μL
– Source: Recombinant E. coli strain
– Optimal reaction temperature: 37 °C
– Heat inactivation: 65 °C for 15 min
– Storage conditions: Store at -20 °C
– Shipping conditions: Shipped at dry ice
Components:
– T4 ssRNA Ligase (10 U/μL)
– Reaction Buffer (10x)