Produkty
Producenci
DNase I Solution (Molecular Biology Grade)
Opis
3 x 10 000u
Description:
DNase I (RNase-free) is an endonuclease that digests ssDNA, dsDNA and DNA in DNA-RNA complexes. The enzyme
activity is strictly dependent on Ca²⁺ and is activated by Mg²⁺ and Mn²⁺ ions.
Enzyme is purified from P. pastoris expressing bovine pancreas DNase I gene. DNase I may be used to degrade DNA
in applications that are sensitive to the presence of RNases.
Applications:
- Degradation of DNA template in transcription reactions
- Removal of contaminating genomic DNA from RNA samples
- DNase I footprinting
- Nick Translation
Components:
- DNase solution (10 U/μl): in 10 mM Tris-HCl (pH 7,5), 2 mM CaCl2, 50% Glycerol (v/v)
- Reaction buffer (10X): 10 x 1,5 ml 500 mM Tris-HCl, pH 8.0, 50 mM MgCl2
Unit definition: One Kunitz unit of DNase I completely degrades 1 μg of plasmid DNA in 10 min at 37°C.
RNase activity: not detected (incubation of RNA transcript with DNase I
Shipping and storage: Shipping on blue ice. Stable at room temperature for up to 7 days.
Stability: 3 years at -20°C
Dane techniczne
| Opakowanie | 30000 U |