Produkty
Producenci
FastGene StuI
Opis
- No Star Activity
- Highest Purity
- Restriction in just 5 minutes with the FastCut buffer
- Perfect use for Cloning experiments
Activity in FastGene Buffer
Methylation sensitivity:
dam methylation: Not sensitive
dcm methylation: Conditionally sensitive
CpG methylation: Not sensitive
ENZYME FINDER
Different Types of restriction enzymes
Based on the structure, cofactor requirements and specificity of cleavage there are four types of restriction enzymes (Types I, II, III, and IV).
Type I restriction enzymes cleaves the DNA at a random location far away from the recognition sequence. These enzymes require both ATP and S-adenosyl-L-methionine to function.
Type II restriction enzymes cleaves the DNA within or near the recognition sequence. These enzymes do not require ATP and are independent from methylase. The type II enzymes are the most useful restriction endonucleases for the daily laboratory work. All our NIPPON Genetics EUROPE restriction enzymes are from type II.
Type III restriction enzymes cleaves DNA about 20 – 25 base pairs away from the recognition sequence. They require both ATP and S-adenosyl-L-methionine to function.
Type IV restriction enzymes cleaves only modified, typically methylated DNA in contrast to the types I-III, which are usually inhibited by methylation.
Ambiguity Codes
N = A or C or G or T (any)
B = C or G or T (not A)
D = A or G or T (not C)
H = A or C or T (not G)
V = A or C or G (not T)
W = A or T (weak)
S = C or G (strong)
R = A or G (purine)
Y = C or T (pyrimidine)
M = A or C (amino)
K = G or T (keto)
Dane techniczne
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