Produkty
Producenci
SgeI
nr kat.:
ER2211
Opakowanie:
250U
Producent:
nr kat.:
ER2211
Opis
The SgeI restriction enzyme recognizes m5CNNG(9/13)^ sites and cuts best at 37°C in its own unique buffer.
5'...m5C N N G (N)9 ↓...3'
3'... G N N C (N)13↓...5'†
Note
DNA methylated by Dcm or CpG methyltransferases will be a substrate for SgeI. Greater than 3-fold overdigestion with SgeI may result in incomplete cleavage. At least two copies of SgeI recognition sequence are required for an efficient cleavage. Amount of the enzyme required for complete digestion of methylated DNA depends on the number of SgeI recognition sites. DNA cleavage products generated by target site cleavage facilitate the nonspecific cleavage by SgeI.
Therefore, optimization of the enzyme amount is recommended for DNA cleavage. pBR322 DNA isolated from E. coli dcm+ strain (#SD0041) can be used as a DNA cleavage efficiency control. SgeI cleaves all six dcm methylated targets on pBR322 DNA.
For methylation sensitivity refer to product specifications.
Dane techniczne
| Opakowanie | 250U |