Produkty
Producenci
T4 β-glucosyltransferase
nr kat.:
EO0831
Opakowanie:
500U
Producent:
nr kat.:
EO0831
Opis
T4 ß-glucosyltransferase (T4 BGT) transfers the glucose moiety of uridine diphosphoglucose (UDP-glucose) to the 5-hydroxymethylcytosine (5-hmC) residues in double-stranded DNA generating ß-glucosyl-5 hydroxymethylcytosine.
Thermo Scientific T4 BGT is specifically formulated for fast reaction times without compromising the reaction efficiency. The enzyme completes 5-hmC glucosylation of 1 µg DNA at 37°C in 15 minutes.
Highlights
- Specific – selectively transfers glucose to the hydroxymethyl moiety of 5-hmC
- Fast – complete glucosylation of 1 µg DNA in just 15 min
- Convenient – supplied with optimized buffer and UDP-glucose.
Applications
- Locus specific detection of 5-hmC
- Enrichment of 5-hmC containing DNA
- Labeling of 5-hmC residues using a radioactive UDP-glucose donor
Includes
- T4 β-glucosyltransferase, 5 U/µL
- 10X Epi buffer
- 10X UDP-glucose
Dane techniczne
| Opakowanie | 500U |