NZYFood gDNA Isolation kit

NZY Food gDNA Isolation kits are designed for the simple and rapid small-scale preparation of highly pure genomic DNA from a wide variety of food samples. The method is spin column silica-based and requires no phenol or chloroform extractions. This kit ensures lysis using Lysis Buffer NFL and guarantees a good recovery yield from complex and processed food matrices such as spices or ketchup.

To remove cellular debris or contaminants the lysis mixtures should be cleared by centrifugation. The clear flow-through is mixed with Binding buffer NFB for optimal binding of DNA to the silica membrane. Then, the DNA is selectively absorbed into the NZYSpin Food Column and others impurities such as proteins and salts are removed during the washing steps.NZY Food gDNA Isolation kit is optimized to isolate up to 10 µg of DNA from up to 200 mg of food samples, depending on the food source used.

Features:

• High genomic DNA quality
• High yields from multiple sample sources
• Silica-membrane technology
• Efficient removal of PCR inhibitors
• Proteinase K included

Applications:
The purified genomic DNA is suitable for use in the most demanding molecular biology applications, including:

• PCR
• Real-time quantitative PCR (qPCR)
• Genotyping
• DNA sequencing
• Multiplex-PCR
• Enzimatic manipulations

Specifications:
Sample material (general):    food samples
Format:    Spin-column
Sample amount:    5-200 mg
Typical yield:    0.1-10 µg
A260/A280:    1.6-1.9
Elution volume:    100 µL
Binding capacity:    50 µg
Preparation time:    approx. 12 min/preparation (excluding sample homogenization and cell lysis)
High-throughput compatibility:    No
Product size:    50 columns
Storage conditions:    Store all components at room temperature
Shipping conditions:    Shipped at room temperature

Components:
– Buffer NFB
– Buffer NFL
– Buffer NFW1
– Buffer NFW2 (concentrate)
– Buffer NFE
– Proteinase Buffer
– Proteinase K
– NZYSpin Food columns
– Collection tubes (2 mL)