FastDNA™ SPIN Kit for Plant and Animal Tissues

• For the isolation of high-quality genomic DNA from plant and animal tissues
• Uses Lysing Matrix D for cell lysis and a silica-based spin filters for purification.

Designed for use with the FastPrep instruments from MP Biomedicals, plant and animal tissues are easily lysed within 40 seconds. These benchtop devices use a unique, optimized motion to homogenize samples by multidirectional, simultaneous impaction with lysing matrix particles.

FastPrep instruments provide an extremely quick, efficient and highly reproducible homogenization that surpasses traditional extraction methods using enzymatic digestion, sonication, blending, douncing, and vortexing.

Samples are placed into 2.0 mL tubes containing Lysing Matrix D, which is 1.4mm ceramic spheres. While almost all samples are easily processed with Lysing Matrix D, additional lysing matrix options are available at www.mpbio.com for more delicate samples or for tough, hardened samples.

Homogenization in the FastPrep instrument with Lysing Matrix D takes place in the presence of sample-specific Cell Lysis Solutions (CLS). For plant tissues, CLS-VF is used in conjunction with a Protein Precipitation Solution (PPS). For all other samples, including human and animal tissues, CLS-TC is used during sample lysis.

For maximum flexibility, all buffers are provided in the kit.

Following lysis, samples are centrifuged to pellet the debris and the lysing matrix.

DNA is purified from the supernatant with a silica-based GENECLEAN® procedure using SPIN filters. Eluted DNA is ready for digestion, electrophoresis, PCR and any other desired application.

Components:

• Lysing Matrix D 100x 2.0 mL tubes,
• 1/4” Ceramic Spheres 100 spheres,
• Binding Matrix 100 mL,
• Concentrated SEWS-M 12 mL,
• DES 25 mL,
• CLS-VF 90 mL,
• PPS 25 mL,
• CLS-TC 110 mL,
• SPIN Modules 100 each,
• Catch Tubes 100 each.