FastRNA™ SPIN Kit for Microbes

The FastRNA™ SPIN Kit for Microbes quickly and efficiently isolates high-quality, total RNA from tough-to-lyse bacterial cell cultures and other microbial sources in approximately 15 minutes using a zirconium silicate Lysing Matrix (0.1mm) to lyse bacteria and SPIN columns for the purification process. The kit is designed for processing samples in 2-ml tubes using the FastPrep-24™ Instrument. Gram(+/-) bacteria, wet, dry, or frozen are easily lysed in approximately 60 seconds.

Isolate both large and small RNA species without the use of phenol or reducing agents. Small RNAs (e.g., tRNAs, microRNAs) can be recovered following a simple adjustment within the RNA isolation protocol. RNA (~5 µg) isolated from 101 to 105 cells can be eluted into volumes as little as 6 µl in less than 15 minutes. High quality total RNA (A260/A280 > 1.8, A260/A230 > 1.8) is recovered. RNA is eluted with RNase-free water and can be stored at =-70 °C. The addition of RNase inhibitors is optional, but highly recommended for prolonged storage.

Components:

• Lysing Matrix B (0.1mm zirconium silicate) 50 x 2-mL tubes,
• FastRNA Lysis Buffer 50 mL,
• FastRNA Prep Buffer 25 mL, FastRNA Wash Buffer 12 mL,
• Water (DNase/RNase-Free) 1 mL,
• FastRNA Spin Column 1 50 each,
• FastRNA Spin Column 2 50 each,
• Collection Tubes 2mL 100 each.