SPINeasy® DNA Kit for Plant

SPINeasy DNA Kit for Plant is a silica-membrane spin-column kit that enables quick, convenient and cost-effective isolation of genomic DNA (gDNA) from various plant tissues, such as leaf, stem and seed. High quality gDNA can be extracted from polysaccharide and polyphenolic-rich plants.

Components: 50 x 2mL Lysing Matrix A, 50mL Lysis Buffer PD, 12mL PPS Solution, 15mL Binding Buffer PD, 2 x 12mL Wash Buffer, 10mL Elution Buffer PD, 0.8mL RNase A, 50 x Column PD with collection tubes.

Sample Type: Plant tissues including plant cells, lichen, rice, grains, leaves, stems, buds, fruit, and seeds; 50 – 150 mg input

The use of our specially formulated Lysis Buffer PD and Lysing Matrix A in combination with FastPrep® Instruments from MP Biomedicals enables highly efficient lysis of various types of samples within seconds. For DNA purification, Column PD and kit buffers are designed to deliver gDNA extracts of high yield and purity which are compatible with downstream molecular biology applications such as PCR, restriction digestion and sequencing.

Kit performance
    

Figure 1 (left): gDNA extracted from various samples using SPINeasy DNA Kit for Plant, analyzed using agarose gel electrophoresis. M: DNA marker; Lane 1: Pothos leaf; Lane 2: Tomato leaf; Lane 3: Chili leaf; Lane 4: Tobacco leaf; Lane 5: Pine needle; Lane 6: Corn leaf; Lane 7: Corn kernel.
       

Figure 2 (right): PCR amplification of gDNA extracted from various samples using SPINeasy DNA Kit for Plant. M: DNA marker; Lane 1: Pothos leaf; Lane 2: Tomato leaf; Lane 3: Chili leaf; Lane 4: Tobacco leaf; Lane 5: Pine needle; Lane 6: Corn leaf; Lane 7: Corn kernel; Lane 8: Negative control
   
Application Notes
Deliver gDNA extracts of high yield and purity from plant tissues that are compatible with downstream applications such as PCR, restriction digestion and sequencing.
Usage Statement
Research Use Only (RUO)

Key Applications
Plant Genomics, Molecular Biology, PCR, NGS