NZY Mag NGS Clean-up & Size Selection

The NZY Mag NGS Clean-up & Size Selection is a cutting-edge paramagnetic bead-based system optimized for rapid and efficient purification of DNA and RNA fragments from enzymatic reactions, as well as precise size selection of DNA during the library construction process for next-generation sequencing (NGS) workflows. This solution is formulated to provide scientists with high recovery yields and exceptional flexibility, addressing common challenges in clean-up and size selection steps. Utilizing NZYtech's proprietary paramagnetic bead suspension in a specialized binding buffer, nucleic acids are selectively bound based on the ratio of beads to sample.

The workflow includes:

• Binding of DNA or RNA to the beads.
• Magnetic separation and removal of contaminants (nucleotides, primers, adapters, dimers, enzymes, salts, and buffer additives).
• Ethanol washes to ensure thorough purification.
• A short drying step to eliminate residual ethanol.
• Elution of highly pure nucleic acids, RNA or DNA, in a low-salt elution buffer or water for immediate downstream applications.

The purified DNA and RNA are suitable for a wide range of molecular biology and sequencing applications, including PCR amplification, cDNA synthesis, RNA-Seq, whole-genome sequencing, and targeted sequencing workflows. The NZY Mag NGS Clean-up & Size Selection was designed for manual or automated purification of DNA and RNA fragments from various enzymatic reaction mixtures.

The NZY Mag NGS Clean-up & Size Selection delivers exceptional recovery and precision, achieving typical yields of > 80% for DNA and > 70% for RNA, while enabling tunable size selection of double-stranded DNA fragments between 150 bp and 800 bp with input amounts ranging from 5 ng to 1 μg. Designed for optimized reproducibility, the system accommodates sample volumes of ≥ 50 μL and operates seamlessly at room temperature, eliminating the need for refrigeration. Its versatility extends to manual workflows and automated liquid-handling systems, offering scalable solutions for high-throughput applications. The resulting highly purified nucleic acids are of unparalleled quality, supporting critical downstream processes such as PCR, qPCR, NGS library preparation, sequencing on all major platforms, in vitro transcription, and other molecular analyses. By combining precision, adaptability, and automation compatibility, the NZY Mag NGS Clean-up & Size Selection ensures superior performance for the demanding clean-up and size selection requirements of modern NGS workflows.