Features:
– Quick maxi-prep of pure plasmid DNA
– Efficient removal of endotoxins and other contaminants in two washing steps
– Silica-based anion-exchange columns
– High yields of plasmid DNA (typically 500 µg/ column)
– Endotoxin Free H2O provided
Applications:
The purified plasmid DNA, free of endotoxins, is suitable for use in:
– Transfection
– In vitro transcription
– Enzymatic reactions
– Automated fluorescent or manual sequencing
– PCR
– Cloning
– Hybridization
Specifications:
| Culture volume/starting material: | 100-250 mL E. coli culture volume |
| Plasmid type: | high- and low-copy number, cosmids or large constructs ranging from 3-300 kbp |
| Format: | gravity flow column |
| Yield: | up to 500 µg |
| A260/A280: | 1.8–1.95 |
| Elution volume: | variable |
| Endotoxin level: | < 0.1 EU/µg |
| Preparation time: | approx. 100 min/2 preps |
| Product size: | 5 columns, 2 x 5 columns |
| Storage conditions: | All kit components can be stored at room temperature; Buffer NML1-EF with RNase should be stored at 4 °C for frequent use |
| Shipping conditions: | Shipped at room temperature |
Components:
-Buffer NML1-EF, 70 mL
-Buffer NML2-EF, 80 mL
-Buffer NML3-EF, 80 mL
-Buffer NMEQ-EF, 50 mL
-Buffer NMW1-EF, 300 mL
-Buffer NMW2-EF, 150 mL
-Buffer NMEL-EF, 100 mL
-Buffer NME-EF, 15 mL
-Endotoxin-Free H2O, 15 mL
-70% Ethanol (conc.), 15 mL
-RNase A, 7 mg
-NZYMaxi-EF Columns, 5 units
-NZYFolded Filters, 5 units
-NZYPlastic Washers, 2 units