Description: NZY Total RNA Isolation kit is designed for the easy purification of total RNA of highest integrity (longer than 200 bases) from a variety of sources such as animal tissues, bacteria cells and cell cultures. This method uses a denaturing lysis buffer containing guanidine thiocyanate, which inactivates cellular RNases, to ensure the recovery of intact RNA molecules. Ethanol is added to provide selective binding of total RNA into the silica membrane column and impurities are efficiently washed away. To prevent the contamination with DNA, a DNase I solution is directly added onto the silica membrane of the binding column. High-quality RNA is then eluted in RNase-free water.
Features:
– High RNA quality
– Ready-to-use Total RNA
– High yields from multiple sample sources (animal tissues, yeast, bacterial cells and cell cultures)
– Optimized protocols for a range of starting materials
– High-speed protocol
– Silica-membrane technology
– DNase I included
Applications:
The purified RNA is suitable for use in the most demanding molecular biology applications, including:
– Reverse transcriptase PCR (RT-PCR)
– Real-time quantitative PCR (qPCR)
– in vitro translation
– cDNA synthesis
Specifications:
| Sample material (general): | tissue, yeast, bacterial cells, cell cultures |
| Format: | Spin-column |
| Sample amount: | up to 30 mg tissue/1 x 109 bacterial cells/5 x 106 cultured cells/108 yeast cells |
| Typical yield: | up to 70 μg |
| A260/A280: | 1.9-2.1 |
| Elution volume: | 40-60 μL |
| Binding capacity: | 200 μg |
| Preparation time: | 35 min |
| High-throughput compatibility: | No |
| Support protocols: | yes (see “Product resources” above) |
| Product size: | 50 columns |
| Storage conditions: | Store all components at room temperature |
| Shipping conditions: | Shipped at room temperature |
Components:
– Buffer NR
– Buffer NI
– Buffer NRW1
– Buffer NRW2 (concentrated)
– RNase free water
– Digestion buffer
– DNase I (lyophilized)
– NZYSpin Homogenization Columns (purple rings)
– NZYSpin Binding Columns (blue rings)
– Collection tubes (1.5 mL and 2 mL)