Produkty
Producenci
Minute™ Ponceau S Stain
Opis
This is a rapid and reversible staining used for locating protein bands on Western blots and comparing protein band intensity. It produces reddish-pink-stained bands on membranes, which is useful because it does not have a deleterious effect on the property of proteins.
It is also commonly used as a method for locating polypeptides for protein sequencing. The stain binds to proteins on the membrane reversibly and works well for nitrocellulose and PVDF membranes. It is not recommended for positively charged membranes such as nylon membranes.
Protocol
1. Incubate the membrane with the stain solution for 1 min to up to 30 min with gentle agitation.
2. Rinse the membrane in deionized water until the background is clean (usually it takes less than
5 min). The stain can be completely removed from the protein bands by continuing washing
with deionized water.
Dane techniczne
| Opakowanie | 250 ml |