Minute™ Bacterial Total Protein Extraction Kit

Invent Biotechnologies Minute™ bacterial total protein extraction kit is composed of optimized denaturing cell lysis buffers and protein extraction filter cartridges with 2.0 ml collection tubes. The kit is designed to rapidly extract denatured proteins from bacteria.
The kit contains sufficient materials for extraction of total proteins from 100 ml E.coli culture.

Application
MinuteTM bacterial total protein extraction kit is designed to rapidly extract total proteins from cultured bacterial cells for applications such as SDS-PAGE, immunoblottings,
ELISA and other applications. This kit provides the most rapid method for preparation of whole bacterial cell extract.
Buffer Formulation: Proprietary

Kit Components
1. 25 ml bacterial lysis buffer A (Part#: SB-004-A)
2. 2.5 ml buffer B (Part#: SB-004-B)
3. 50 protein extraction filter cartridges (Part#: P-001)

4. 50 collection tubes with cap (Part#: P-002)

Lane 1: E. coli proteins prepared by sonication; Lane 2 and 3: E. coli proteins prepared by Minute™ Bacterial Total Protein Extraction Kit (#SB-004)

Important Product Information

The Minute™ bacterial total protein extraction kit is designed to extract total protein rapidly from bacteria. The use of protease inhibitors is not necessary prior to extraction.

However if downstream application takes significant amounts of time or the protein extract will be stored for longer period of time, addition of protease inhibitor to extracted lysate is recommended. For determination of protein concentration BCA kit is recommended. The protein extraction filter cartridge has the capacity of 500 μl. Multiple filter cartridges may be used if larger amount of cell lysate is desired. To study protein phosphorylation, phosphatase inhibitors must be added to lysis buffer prior to use.

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