Minute™ Cytoplasmic and Nuclear Fractination Kit

Invent Biotechnologies Minute™Cytolpasmic and Nuclear Extraction Kit is composed of optimized cytoplamic extraction buffer, nuclear protein extraction buffer and protein extraction filter cartridges with 2.0 ml collection tubes. The kit is designed to rapidly separate native cytosol and nuclear proteins from cultured mammalian cells or tissues.
Due to the use of the protein extraction filter cartridges separation of cytoplasmic and nuclear proteins can be accomplished in less than 15 min.

Application
The kit is designed to rapidly extract native cytoplasmic and nuclear proteins from cultured cells or tissues for applications such as SDS-PAGE, immunoblottings, ELISA, IP, protein localization, gel mobility shift assays, 2-D gels and other applications. This kit provides the most rapid method currently available for fractionation of native cytoplasmic and nuclear proteins.

Buffer Formulation: Proprietary

Kit Components
1. 25 ml cytoplamic extraction buffer (Part#: SC-003-L)
2. 25 ml nuclear extraction buffer (Part#: SC-003-N)
3. 50 protein extraction cartridges (Part#: P-001)
4. 50 collection tubes with cap (Part#: P-002)

Storage
Store the kit at 4^oC upon arrival.
 

Important Product Information
The use of protease inhibitors is not necessary prior to extraction. However if downstream application takes significant amounts of time or the protein extract will be stored for longer period of time, addition of protease inhibitor to extracted lysate is recommended. The nuclear extraction buffer contains 300 mM salt, for some applications, dilution or desalting of the extract may be needed. For determination of protein concentration, BCA kit is recommended. The protein extraction filter cartridge has the capacity of 500 μl. Multiple filter cartridges may be used if larger amount of cell lysate is desired. To study protein phosphorylation, phosphatase inhibitors must be added to lysis buffer prior to use.

Lane 1 and 2: 293T cell cytosol and nuclear fractions (name-brand kit); Lane 3 and 4: cell cytosol and nuclear fractions (Minute™ Cytoplasmic & Nuclear Extraction Kit, #SC-003)

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