Minute™ Detergent-Free Total Protein Extraction Kit Animal Cultured Cells/Tissues

Invent Biotechnologies Minute™ detergent-free total protein extraction kit is composed of optimized cell lysis buffer and protein extraction filter cartridges with 2.0 ml collection tubes. The kit is designed to rapidly extract total proteins from cultured cells (insect/mammalian/other cultured cells) and animal tissues (invertebrate and vertebrate). The protein extraction buffers do not contain any detergent and EDTA. Due to the use of the protein extraction filter cartridges, the extraction volume can be as small as 20 μl and as large as 500 μl. This unique feature is very useful in situations where available starting material is a limiting factor. Detergent-free total proteins can be extracted from cultured cells/tissues in less than 5 min with high yield (1-5 mg/ml).

Application
Minute™ detergent-free total protein extraction kit is designed to rapidly extract total proteins from invertebrate and vertebrate cultured cells/tussues for applications such as proteomics (LC/MS), IP, ELISA, 2D-gel analysis, isoelectric focusing, SDS-PAGE, immunoblottings, and other applications. This kit provides a very rapid method for preparation of high concentration of whole cell extract. Because of high yield of protein extract and low salt concentration, the protein extract can be used directly for downstream applications without desalting and protein concentration.

Comparison of protein profiles of different animal tissues extracted by denaturing lysis buffer and detergent-free extraction buffer.
Lane 1: Drosophila cultured cells (SL2); Lane 2: Drosophila adult flies; Lane 3: gold fish muscle; Lane 4: mouse liver. Extracted proteins were separated in 10% SDS-PAGE and stained with coomassie blue.

Kit components
1. 15 ml buffer A
2. 15 ml buffer B
3. 50 protein extraction filter cartridges
4. 50 collection tubes with cap
5. Plastic rods (4)

The Minute™ total protein extraction kits are designed to extract total protein rapidly. The use of protease inhibitors is not necessary prior to extraction. However if downstream application takes significant amounts of time or the protein extract will be stored for longer period of time, addition of protease inhibitors to buffer A is recommended. For determination of protein concentration, BCA kit is recommended. To study protein phosphorylation, phosphatase inhibitors should be added to buffer A prior to use.

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