
MobiTEVProtease1 (HighSpeed), recombinant, His-Tag
Opis
Description
MobiTEV Protease1 (HighSpeed) is used to remove fusion tags -solubility, secretion, detection, and purification tags -and to release the "native" protein of interest. MobiTEV Protease1 (short: MobiTEV1) is an improved version of Tobacco Etch Virus (TEV) protease that is highly site-specific, highly active, and significantly more stable than native TEV Protease, resulting in enhanced long-term activity.
MobiTEV1 specifically recognizes a seven amino acid sequence (Glu-Asn-Leu-Tyr-Phe-Gln-Gly, cleaving between Gln and Gly). The MobiTEV1, is fused to a 6xHis-Tag and can be removed from the reaction mixture by Ni-IDA or Ni-NTA chromatography.
Source
The DNA sequence encoding a mutant form of nuclear inclusion protein a (NIa) protease from tobacco etch virus (TEV Protease) was fused to a hexahistidine tag. To avoid proteolytic auto-inactivation, the serine amino acid residue at position 219 was replaced by valine.The chimeric protein was expressed in E. coliBL21(DE3) and S219V TEV Protease mutant was purified on a Ni-NTA column.
Dane techniczne
Opakowanie | 10 x 1000 U |