Produkty
Producenci
CSM-TRP (POWDER)
nr kat.:
114511012
Opakowanie:
10 g
Producent:
nr kat.:
114511012
Opis
Single dropout formulation ( without L-Tryptophan) of Complete Supplement Mixture (CSM) of amino acids for S. cerevisiae growth media.
| Formulation Details | Contents per Liter: Adenine 10* mg |L-Arginine HCl 50 mg |L-Aspartic Acid 80 mg |L-Histidine HCl 20 mg |L-Isoleucine 50 mg |L-Leucine 100 mg |L-Lysine HCl 50mg |L-Methionine 20** mg |L-Phenylalanine 50 mg |L-Threonine 100** mg |L-Tryptophan 0 mg |Uracil 20 mg| L-Tyrosine 50 mg |L-Valine 140 mg |Total 740.000 |*Minimum quantity for healthy growth and yet optimized to promote red color in certain adenine auxotrophs. CSM formulations are available that contain 20 or 40 mg/liter adenine. **80mg/liter of DL-Homoserine is substituted for L-Threonine in mixtures where L-Methionine is dropped out. |
| Application Notes | The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. This CSM-Dropout medium create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs. |
Dane techniczne
| Opakowanie | 10 g |