YPD (YEPD) BROTH (PACK)(POUCHES)

YPD (YEPD) is a nutritionally rich medium for the growth of S. cerevisiae, containing dextrose, peptone and yeast extract. Pre-measured, sealed pouches, for 0.5 L or 1 L batch sizes, simply tear open the pouch and add to water.

Application Notes

YPD Broth is used for routine growth of Saccharomyces cerevisiae for molecular biology studies.

Format: Pouch

Formulation Details     

Contents Per Liter: 20 g Peptone Y; 10 g Yeast Extract-Y; 20 g Dextrose. Small quantities of biological buffers have been added for proper pH adjustment.
Grade: Molecular Biology Certified™
Media Type: YPD

pH: Acceptable pH range is 6.2 ± 0.3 at 25 °C

Preparation Method     

Media Preparation: Reagents are tested and chosen for their ability to promote optimum growth of S. cerevisiae when combined in YPD Broth formulations. Chosen reagents are combined in the proper proportions according to the YPD formulation and thoroughly blended for a uniform distribution. A sample of the blended formulation is used to prepare liquid medium. The combinations are mixed to dissolve components and then autoclaved at 121 °C for 15 minutes.

Solubility: Soluble in water (25 g/0.5 L).

Sterilization of Solutions:     Combinations are mixed to dissolve components and then autoclaved at 121 °C for 15 minutes. Note: Autoclaving at higher temperatures or for longer periods will result in darkening of the sterile medium, (a darker color does not normally affect growth of cells, only the appearance of the sterile medium).

Typical Working Concentration: 50 g/L