Produkty
Producenci
Hygromycin B Concentrated
Opis
Produkt przeznaczony wyłącznie do badań. Nie nadaje się do spożycia.
Hygromycin B is a unique aminoglycoside antibiotic derived from Streptomyces hygroscopicus. Hygromycin B is routinely used as a selective agent in cell culture or microbiology applications to isolate hygromycin B resistant cells.
This product is packaged as a concentrated solution containing 350-450 units/mL.
CAS Number: 31282-04-9
Molecular Formula: C20H37N3O13
Molecular Weight: 527.52 g/mol
Mechanism of Action
Hygromycin B inhibits protein synthesis by strengthening the interaction of tRNA binding in the ribosomal A-site. Hygromycin B also prevents mRNA and tRNA translocation by an unknown mechanism. These are unique mechanisms for an aminoglycoside antibiotic and they differ from the mode of action neomycin, gentamicin, and G418.
Spectrum
Hygromycin B is effective against eukaryotic and prokaryotic cells.
Eukaryotic Cell Culture Applications
Hygromycin B is routinely used as a selective agent in mammalian cell culture to isolate hygromycin B resistant cells after transfection. Selectable markers for hygromycin B resistant cells include the hyg or hph resistance genes which express a phosphotransferase that inactivates hygromycin B by phosphorylation. Effective working concentrations range from 100 – 1000 µg/mL.
For additional information regarding relevant cell lines, resistance plasmids, and culture media, please visit our cell culture database.
Microbiology Applications
Hygromycin B can be used as a selection agent to isolate hygromycin b resistant bacteria and fungi.
Source: Biosynthetic: produced by Streptomyces hygroscopicus.
Density: 1,100 - 1,600 mg/mL
Dane techniczne
| Opakowanie | 1 MU |