DharmaFECT Duo Transfection Reagent
Opis
In side-by-side cotransfection experiments with traditional reagents developed for plasmid delivery, only DharmaFECT Duo Transfection Reagent resulted in efficient cell delivery of both siRNA (assayed by target gene knockdown) or microRNA reagents, along with plasmid (assayed by level of reporter expression). DharmaFECT Duo is an excellent choice for experiments requiring cotransfection, such as RNAi rescue or utilization of reporter systems.
Highlights
- Effective delivery of two distinct molecules, DNA plasmid and small RNA, from a single formulation
- Efficient transfection without compromising cell viability
- Potent RNAi results together with plasmid expression
For transfection of plasmid DNA or shRNA, please select DharmaFECT kb Transfection Reagents. For transfection of siRNA or microRNA only, please select DharmaFECT 1 Transfection Reagent.
DharmaFECT Duo transfection reagent achieves reliable cotransfection results for a variety of experimental goals. The graph demonstrates specific modulation of Renilla luciferase (Rluc) gene:
- Rluc expression is similar for Plasmid Alone and Plasmid+Control
- Down-regulation of the Rluc as a result of miRNA mimic (plasmid + miRNA mimic)
- Excellent knockdown of the Rluc by siRNA (Plasmid + siRNA)
The psiCHECK-2 vector (100 ng/well; Promega) with cloned microRNA recognition sites for miR-28, miR-95, miR-30d, and miR-105, respectively, were complexed alone or with RNAi reagents (10 nM) using DharmaFECT Duo (0.2 μL/well) in MCF-7 cells at 10,000 cells/well (96-well plate). The RNAi reagents were Dharmacon miRIDIAN Mimics (Negative Control, miR-28, miR-95, miR-30d, and miR-105, respectively) or siRNA (Renilla luciferase pool). Firefly and Renilla luciferase expression was assessed at 48 hours using the Dual-Glo Luciferase Assay System (Promega) and normalized to identically treated psiCHECK-2 empty vector.
DharmaFECT Duo for Simultaneous Delivery of RNA and DNA
Citations
- X. Guanlan et al., Preventing β-Cell Loss and Diabetes With Calcium Channel Blockers. Diabetes. 61(4), 848-856 (Apr 2012).
- Y. Wang et al., Suppression of GSK-3β activation by M-cadherin protects myoblasts against mitochondria-associated apoptosis during myogenic differentiation. J. Cell Sci. 124, 3835-3847 (November 2011).
- X. Wang et al., Characterization of Promoter Elements Regulating the Expression of the Human Neurotensin/Neuromedin N Gene. J. Biol. Chem. 286(1), 542-554 (7 January 2011).
- S. Sinha et al., Von Hippel-Lindau Gene Product Modulates TIS11B Expression in Renal Cell Carcinoma: Impact On Vascular Endothelial Growth Factor Expression In Hypoxia. J. Biol. Chem. 284(47), 32610-32618 (20 November 2009).
- A. Poleshko et al., Identification of Cellular Proteins That Maintain Retroviral Epigenetic Silencing: Evidence for an Antiviral Response. J. Virol. 82(5), 2313-2323 (March 2008).
Dane techniczne
Opakowanie | 0,75 mL |