Produkty
Producenci
T4 RNA Ligase
nr kat.:
EL0021
Opakowanie:
1000 U
Producent:
nr kat.:
EL0021
Opis
Thermo Scientific T4 RNA Ligase catalyzes the ATP-dependent intra- and intermolecular formation of phosphodiester bonds between 5'-phosphate and 3'-hydroxyl termini of oligonucleotides, single-stranded RNA and DNA.
The minimal substrate is a nucleoside 3',5'-biphosphate in intermolecular reaction and oligonucleotide of 8bases in intramolecular reaction.
Applications
- RNA 3'-end labeling with cytidine 3',5'-bis [alpha-32P] phosphate
- Joining RNA to RNA
- Synthesis of oligoribonucleotides and oligodeoxyribonucleotides
- Specific modifications of tRNAs
- Oligodeoxyribonucleotide ligation to single-stranded cDNAs for 5' RACE (Rapid Amplification of cDNA Ends)
- Site-specific generation of composite primers for PCR
| Specification: | |
| 10X Reaction Buffer | 500mM Tris-HCl (pH7.5 at 25°C), 100mM MgCl2, 100mM DTT, 10mM ATP |
| Concentration | 10 U/µL |
| Definition of Activity Unit | One unit of the enzyme catalyzes the conversion of 1nmol of 5'-[32P]-(A)12-18 to a phosphatase-resistant form in 30min at 37°C. Enzyme activity is assayed in the following mixture: 50mM Tris-HCl (pH7.5), 10mM MgCl2, 10mM DTT, 1mM ATP, 10µM 5'-[32P]-(A)12-18 (10µM in 5'-termini). |
| Inactivation | Inactivated by heating at 70°C for 10min. |
| Inhibition | Inhibitors: metal chelators, SH group-modifying reagents |
| Molecular Weight | 43.6kDa monomer |
| Quality Control | The absence of ribonucleases, exodeoxyribonucleases, endodeoxyribonucleases, and phosphatases confirmed by appropriate quality tests. |
| Source | E.coli cells with a cloned gene 63 of bacteriophage T4 |
| Storage Buffer | Th enzyme is supplied in 20mM Tris-HCl (pH7.5), 1mM DTT, 50mM KCl, 0.1mM EDTA, 0.03%(v/v) ELUGENT Detergent and 50%(v/v) glycerol. |
Dane techniczne
| Opakowanie | 1000 U |