FastGene Scriptase Basic cDNA Kit

Optimized for better performance

The FastGene® Scriptase Basic is an enhanced version of the Murine Leukemia Virus (MuLV) reverse transcriptase. Hence, like the wildtype, it has the ability to synthesize a cDNA strand, a reduced RNase H activity and processitivity. The robustness however was greatly increased. It is the perfect enzyme for large RNA quantities and easy templates.

Applications

• Quantification of Gene Expression
• Endpoint RT-PCR
• High RNA concentrations

No inhibition – Even at large concentration

The special buffer formulation permits a high RNA concentration. Other reverse transcriptases are not able to process such large quantities.
Designed for Endpoint RT-PCR

The FastGene® Scriptase Basic was designed for large RNA quantities, typically used in an endpoint RT-PCR. Nonetheless, it is also able to process lower RNA concentrations.
Enzyme only or cDNA Synthesis kit

The FastGene® Scriptase Basic is available as enzyme only, containing the enzyme, buffer and dNTPs as well as a
cDNA synthesis kit which contains the components of the enzyme plus Oligo dTs, random hexamers and RNase inhibitor. We have also chosen a 100 reactions kit size so that you have enough for a whole 96-well plate.
Comparison of FastGene Scriptase Basic and a conventional MuLV

(A) Competitor N                                        (B) FastGene® Scriptase Basic

RNA (ng)             2       1       0.5    0.1    0.01                            2        1       0.5    0.1    0.01


Fig. 1: Higher sensitivity of the FastGene® Scriptase Basic.
Compared to a wildtype MuLV (A) the FastGene® Scriptase Basic (B) is an optimized MuLV-Reverse transcriptase which has a higher sensitivity, being able to produce a template from RNA concentrations as low as 0.1 ng.

Kit components:

• FastGene®Scriptase Basic (200 U/μl)
• 10x FastGene® Scriptase Basic buffer
• dNTP Mixture (2 mM each)
• Sterile water (RNase free)
• RNase Inhibitor (40 U/μl)
• Oligo dT (80 μM)
• Random Hexamer(100 μM)