Description: NZY First-Strand cDNA Synthesis Flexible Pack provides robust transcription of RNA as it includes all the necessary components to synthesize first-strand cDNA from an RNA template.
This optimized pack includes the following components: our modified recombinant form of the Moloney Murine Leukemia Virus (M-MuLV) Reverse Transcriptase (RNase H minus) that synthesizes the complementary DNA strand using either RNA or single-stranded DNA as template in a wide range of temperatures (37-50°C); an optimized Reverse Transcriptases buffer; a NZY Ribonuclease Inhibitor for RNA protection against degradation due to ribonuclease contamination; a dNTPs mix; random hexamers and oligo(dT)18 primers; and finally, an E. coli RNase H to specifically degrade the RNA template in cDNA-RNA hybrids after the first-strand cDNA synthesis.
This flexible pack offers the possibility to choose the primer to initiate the reaction: you can choose either the primers provided in separate tubes, or a gene-specific primer. The pack is sufficient for up to 200 first-strand cDNA synthesis reactions and it is optimized for RT reactions over a wide range of total RNA concentrations from 10 pg to 5 μg. Downstream applications include real-time PCR, standard PCR and microarrays.
Features:
– Provides high yields of full-length cDNA products
– Formulated to increase sensitivity in RT-qPCR
– Primer type: oligo(dT)18 and random hexamers
– Starting material: 10 pg to 5 µg of total RNA
– Thermostable – working temperature range 37-50 ºC
– Convenient and reliable
Applications:
– First-strand cDNA synthesis for use in real-time quantitative RT-PCR (RT-qPCR)
– Two-step RT-PCR assays
– Ideal for high GC-content templates due to the optimum activity at 50 ºC
Specifications:
– Storage conditions: Store all kit components at -20 ºC
– Shipping conditions: Shipped with dry ice
Components:
– NZY Reverse Transcriptase (200 U/μL)
– 10x Reaction buffer for Reverse Transcriptases
– NZY Ribonuclease Inhibitor (40 U/µL)
– dNTP Mix (10 mM each)
– Random hexamer mix (50 ng/µL)
– Oligo(dT)18 primer mix (50 μM)
– NZY RNase H (E. coli) (5 U/μL)
– DEPC-treated H2O