Producenci
Supreme NZY First-Strand cDNA synthesis Kit, separate oligos
Opis
Supreme NZY First-Strand cDNA Kit is an all-in-one solution, engineered to deliver unmatched efficiency and speed for first-strand cDNA synthesis. Its key features include:
- Rapid cDNA Synthesis: Enables efficient first-strand cDNA synthesis in just 1 minute, significantly reducing reaction time without compromising yield or quality;
- Broad Temperature Compatibility: Optimized for use across a wide range of temperatures (30–70°C), with a recommended reaction temperature of 60°C for enhanced efficiency and robustness;
- Superior Performance: Designed to deliver high yields of full-length cDNA, even with low RNA input amounts, supporting downstream applications with unmatched accuracy and sensitivity;
- Streamlined Workflow: Provided as a ready-to-use 5x master mix and an enzyme mix, accelerating cDNA synthesis workflow while reducing potential errors;
- Versatile Applications: Ideal for a variety of reverse transcription assays, including real-time PCR, RNA quantification, and gene expression studies;
| Application | cDNA synthesis, RT-PCR, RT-qPCR |
|---|---|
| Features | Fast, High Accuracy and Processivity |
| Format | Kit, Liquid |
| Product Category | cDNA Synthesis |
| Reaction Temperature | 60 °C |
| Heat Inactivation | 85 °C |
| Protocol time | Approx. 30 min. |
Components:
- Supreme RTase Enzyme Mix
- Supreme RTase Master Mix 5x
- 10x Annealing Buffer
- Random hexamer mix
- Oligo (dT)18 primer mix
- RNase H (E. Coli)
- DEPC-treated H2O
Dane techniczne
| Opakowanie | 50 react. |