Produkty
Producenci
Pfu/Psp DNA Polymerase (2X Pre-mix, ready to use)
Opis
Pfu DNA polymerase 2X-preMix is a convenient Mixture of proof-reading Enzyme, reaction buffer dNTP´s and MgCl2. It generated PCR fragments exhibits the lowest error rate of any thermostable DNA polymerases. The fragements are blunt-ended for direct ligation.
Pfu/Psp DNA polymerase replicates DNA at 75°C catalyzing the polymerization of nucleotides into duplex DNA in the 5´→3´ direction in the presence of Mg+. Pfu DNA polymerase possesses 3' to 5' exonuclease proof reading activity that enables the polymerase to correct nucleotide-misincorporation errors. To reduce the risk of contamination, pipetting errors and to increase the repeatable of results the 2X-preMix contains an optimized mixture of enzyme, dNTP's and reaction buffer. Just add your template DNA and primers.
Applications:
- Blunt end PCR cloning
- PCR and primer extension where "high fidelity" is required
- Site-directed mutagenesis
- PCR where visual control is needed
Description:
Pfu/Psp DNA polymerase 2X-preMix is isolated from the archae bacteria Pyrococcus f-species, a thermostable Polymerase of approximately 90000 daltons. Base misinsertions that may occur during polymerization are rapidly excised by the proofreading activity of the polymerase. The Pfu/Psp DNA Polymerase has no detectable reverse transcriptase activity.
Concentration: Premix 2X (25µl per reaction)
Quality control:
- Tested for the DNA amplification of 2,2 kb from lambda DNA
- Contamination level check of bacterial DNA
- Purity by SDS-Page > 90 %
Usage:
Standard protocol:
Do not use dUTP or dITP or primers containing these nucleotides
Dane techniczne
| Opakowanie | 100 react. |