NZYLong 2x Green Master Mix

NZYLong 2× Green Master Mix is a premixed ready-to-use solution containing NZYLong DNA polymerase (MB003), an engineered DNA polymerase designed to amplify long target DNA sequences, generally of 20 kb and beyond. A wide range of long PCR products can be generated using lambda DNA or human genomic DNA as starting template. The master mix contains dNTPs, reaction buffer and additives at optimal concentrations for the efficient amplification of an extensive range of DNA templates. MgCl2 final concentration is 2.5 mM, allowing the implementation of a variety of PCR protocols. In addition, reactions assembled with NZYLong 2× Green Master Mix may be directly loaded onto agarose gels. There are two dyes (blue and yellow) in the mix that allow monitoring the progress of electrophoresis.

NZYLong DNA polymerase generates a mixture of A-overhang-ended (predominantly) and blunt-ended PCR products, being suitable for cloning with NZYTech´s TA PCR cloning kits (MB053 or MB137).

Features:

• Convenient ready-to-use PCR master mix
• Direct Gel Loading
• Amplifies long DNA fragments (up to 20 kDa)
• Higher fidelity than NZYTaq DNA polymerase

Applications:

• Long-Range PCR
• Colony PCR
• Generation of products for TA cloning

Convenience of direct gel loading of different-sized PCR products:
The convenience of NZYLong 2x Green Master Mix can be demonstrated through an high-throughput PCR experiment with 96 genes of different sizes (from around 0.5 to around 5 kb).

Specifications:

• Product length:    0-20 kb
• Hot-start-like capacity:    no
• Extension time:    60 sec/kb at 68 °C
• 3´→5´ activity:    no
• Product overhang:    mixed
• Storage conditions:    Store at -20 ºC
• Shipping conditions:    Shipped at 4 ºC to dry ice

Components:
NZYLong 2× Green Master Mix (0.2 U/μL)