FAST 2.0 Bst Mastermix (2x) with SybrGreen for LAMP – Loop-mediated Isothermal Amplification

Fast Isothermal Amplification for sensitive detection in less than 10 minutes

FAST 2.0 Bst Mastermix (2x), genetically improved Bacillus stearothermophilus cloned to E. Coli in a ready-to-use Mastermix, for next generation of isothermal DNA amplification.
The Mastermix allows the detection within 5 to 10 minutes (when using an extra primer pair) and thus it is 2 to 3 times quicker than other Bst DNA Polymerases.

The optimized FAST 2.0 Bst Mastermix amplification results can be compared with about 28-31 cycles in a standard PCR-Cycler Platform. The FAST 2.0 Bst Mastermix offers high strand displacement capability. The optimal temperature range is between 60°C and 65 °C.
Benefits: - Can be more resistant in Plant tissue of blood samples to inhibitors - Very fast screening from minimal processed individual DNA samples - Inexpensive and simple method - Extreme sensitive detection

Applications: FAST Bst. DNA is an extraordinary candidate for isothermal amplifications:
- High strand displacement amplification
- Loop-mediated isothermal amplification of DNA (LAMP)

Content:
Fast 2.0 Bst Polymerase, dNTPs, reaction buffer, glycerol, SybrGreen as intercalator dye, stabilizer and enhancer.
READY-to-use. The Mastermix is 2x concentrated

2 x 1,25 ml (100 reactions/50 μl reaction)

The Mastermix can be combined with ROX reference dye in real time PCR machines working with ROX signal

Storage: @ -20°C for long term; for short term (up to 10-12 weeks) at +4°C

Primer design: To mark 6 DNA regions, usually 4 separate primers are used. To reduce the amplification time, even an additional primer pair can be designed. We recommend to use a professional primer design software. “In-Silco” primer design shall be carefully compared with real existing primer pairs.

Assay: We recommend to work in extremely clean and separate areas for setting up and amplification to reduce the risk of carry-over contaminations. Unspecific products may arise too, when primer dimers of unspecific annealed primers occur.