dNTP/dUTP Mix

Features

Greater than 99% purity confirmed by HPLC.
Free of human and E.coli DNA.
Highly stable – the neutral pH of nucleotide solutions ensures the stability during long-term storage:
– stable for a minimum 100 freeze-thaw cycles;
– 90-95% of dNTPs remain in triphosphate form even after 7 weeks at room temperature;
– 85-90% of dNTPs remain in triphosphate form after 30 cycles of PCR (1 min at 94°C; 3 min at 72°C).

Application tested in:
– cDNA synthesis and RT-PCR,
– real-time PCR,
– standard PCR.

Applications

For use in all molecular biology applications including PCR, real-time PCR & RT-PCR with Taq DNA Polymerase, cDNA synthesis, MDA and DNA sequencing.

Description
PureExtreme® dNTPs are manufactured at Fermentas, one of a few primary manufacturers in the industry. They are supplied in aqueous solutions titrated to pH 7.0 with NaOH. dNTPs are available individually as nucleotide mixes at 2 mM of each dATP, dCTP, dGTP and 4 mM of dUTP for direct use in PCR with Taq DNA Polymerase, reverse transcription and other applications.

Quality Control
Functionally tested in PCR.
Greater than 99% purity of each component confirmed by HPLC.
Free of endo-, exodeoxyribonuclease, ribonuclease and nicking activities.

Note
dNTP/dUTP Mix is not suitable for PCR with proofreading enzymes or enzyme mixes containing proofreading enzymes.

Storage
Store at -20°C.