Supreme NZYTaq II DNA Polymerase

Supreme NZYTaq II DNA polymerase is an engineered version of NZYTaq II DNA polymerase displaying a hot-start-like PCR capacity. The enzyme activity at room temperature is limited, avoiding extension of non-specifically annealed primers or primer-dimers and thus providing higher specificity, sensitivity and yield during DNA amplification. The functional activity of the enzyme is restored during a short 5-minute incubation step at 95 °C. Once Supreme NZYTaq II DNA polymerase is activated, it displays high performance leading to high DNA yields in shorter extension times (15-30 s/kb) of DNA templates up to 6 kb.

Supreme NZYTaq II DNA polymerase requires minimal optimization. In addition, the hot-start-like capacity of Supreme NZYTaq II DNA polymerase not only leads to higher PCR sensitivity but also allows a room-temperature reaction setup. Supreme NZYTaq II DNA polymerase lacks 3´→5´ exonuclease activity. Thus, resulting PCR products have an A-overhang and are suitable for cloning with NZY-A PCR cloning kit (MB053) or NZY-A Speedy PCR cloning kit (MB137).

Features:
– Reproducible and convenient (provides the convenience of room temperature reaction set-up)
– High sensitivity
– Eliminates nonspecific amplifications and minimizes primer-dimers
– Robust amplification using a wide range of DNA templates up to 6 kb
– Leaves an A-overhang

Applications:
– Powerful PCR
– Amplification of low-copy templates
– High specificity amplifications
– Hot-start PCR
– Genotyping
– Generation of products for TA cloning

Specifications:

Components:
– Supreme NZYTaq II DNA polymerase (5 U/μL)
– Reaction buffer (10x)
– MgCl₂ Solution (50 mM)