

phi29 DNA Polymerase
Opis
Supplied with: 1 ml of 10X Reaction Buffer
Concentration: 10 u/µl, (0.1 µg/µl)
.
Thermo Scientific phi29 DNA Polymerase is a highly processive polymerase (up to more than 70 kb) featuring strong strand displacement activity, which allows for highly efficient isothermal DNA amplification (see Reference 1). phi29 DNA Polymerase also possesses a 3'→5' exonuclease (proofreading) activity acting preferentially on single-stranded DNA (see Reference 2) or RNA (see Reference 3). Therefore 3'-modified primers are highly recommended (see Reference 4).
- Highest processivity and strand displacement activity among known DNA polymerases – more than 70 kb long DNA stretches can be synthesized (see Reference 1)
- Highly accurate DNA synthesis (see Reference 5)
- Extremely high yields of amplified DNA even from minute amounts of template
- Amplification products can be directly used in downstream applications (PCR, restriction digestion, SNP genotyping, etc.)
- Rolling circle amplification (RCA) (see Reference 6): generation of periodic DNA nanotemplates (see Reference 7)
- Multiple displacement amplification (MDA) (see Reference 8)
- Unbiased amplification of whole genome (WGA, see Figure 1 in Supporting Data):
- DNA template preparation for sequencing
- Protein-primed DNA amplification (see Reference 15)
- In situ genotyping with padlock probes (see Reference 17)
- Recombination based-cloning (see Reference 18)
- Cell-free cloning of lethal DNA (see Reference 19)
- RNA-primed DNA amplification (see Reference 16)
Dane techniczne
Opakowanie | 1000 U |