

T4 DNA Polymerase
Opis
Thermo ScientificT4 DNA Polymerase, a template-dependent DNA polymerase, catalyzes 5'-3' synthesis from primed single-stranded DNA. The enzyme has a 3'-5' exonuclease activity, but lacks 5'-3' exonuclease activity.
Highlights
- Stronger 3'-5' exonuclease activity on single-stranded than on double-stranded DNA and greater (more than 200 times) than DNA
- polymerase I, E. coli,and Klenow fragment
- Active in Thermo Scientific restriction enzyme, PCR, RT and T4 DNA Ligase buffers
Applications
- Blunting of DNA ends: fill-in of 5'-overhangs or/and removal of 3'-overhangs
- Blunting of PCR products with 3'-dA overhangs
- Synthesis of labeled DNA probes by the replacement reaction
- Oligonucleotide-directed site-specific mutagenesis
- Ligation-independent cloning of PCR products
Dane techniczne
Opakowanie | 100 U |