Maximo Taq DNA Polymerase

nr kat.: S102

Opakowanie: 5 x 500 U

Cena brutto: 661,86 zł 661.86

Cena netto: 538,10 zł

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nr kat.: S102

Opis

Maximo Taq Polymerase is a highly purified polymerase for routine amplification

Maximo Taq DNA Polymerase provides robust PCR performance in a wide range of PCR applications and different templates. Best value in terms of cost per unit.

Applications

Standard / General PCR
Multiplex PCR
High-throughput PCR
Primer extension
Gene mutation
T/A cloning

Maximo Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity and a double-strand specific 5´→3´ exonuclease activity. The enzyme consists of a single polypeptide with a molecular weight of 94kDa.

Concentration: 5 u/µl

Storage Buffer:
25mM Tris-HCl (pH8.0), 100mM KCl, 0.1mM EDTA, 1mM DTT, 50% Glycerol, 0.5% Nonident P40, 0.5% Tween 20

Reaction Buffers supplied with the enzyme:

10X Buffer I: 500mM KCl, 100mM Tris-HCl, pH 9.0, 1% Triton X-100, 15mM MgCl₂

100M MgCl2

Quality control:
- PCR with various templates – genomic DNA, Phage Lambda DNA
- 3 kb DNA amplification from 50 ng DNA
- batch variation and level of bacterial DNA contamination

Storage: at -20°C for 24 months