Minute™ Single Nuclei and Cytosol Isolation Kit for Adipose Tissues/Cultured Adipocytes

Adipocytes are the major energy storage sites in the body and they also have critical endocrine functions. Therefore, understanding the development and function of adipocytes is essential to understanding metabolic homeostasis under physiological and pathological conditions. Fractionation of cellular components into nuclear and cytosolic fractions is a common practice in the lab. However, when it comes to adipocytes, separation of these two fractions is much more difficult because of the high concentration of lipid droplets and low protein content of adipocytes. Methods reported in the literature are tedious and time-consuming and as much as 50 grams of tissue are required. This kit provides a very rapid and simple method for obtaining high purity single nuclei from adipose tissues, and most important of all, only milligram amounts of tissues are required, making it possible to isolate nuclei and cytosol from small animals and biopsy samples. The intact nuclei isolated have many applications such as single nucleus RNA sequencing (snRNA-seq), ATAC-seq, SDS-PAGE, immunoblotting, ELISA, IP, protein localization, gel mobility shift assays, 2-D gels, and other applications.

For storage and transportation of isolated single nuclei, the following buffer is recommended:

WA-014: Minute™ Anti-Clumping Nuclei Storage Buffer 

NOTE: We also offer the following single nucleus isolation kits depending on the tissue types and resistance to exposure of detergent:

NI-024: Minute™ Detergent-Free Single Nuclei Isolation Kit

BN-020: Minute™ Single Nucleus Isolation Kit for Neuronal Tissues/Cells

SN-047: Minute™ Single Nucleus Isolation Kit for Tissues/Cells