LB Broth (granulated)

Luria Broth (Miller’s LB Broth) is based on LB Medium as described by Miller. It is suitable for the growth and maintenance of E. coli strains used in molecular microbiology procedures.

Purified agar with excellent properties for tissue culture and microbiological work. The granulated presentation allows convenient weighing and handling without the mess of powders. Agar is a natural hydrocolloid extracted from several species of red algae, mainly the Gelidium, Gracilaria and  Pterocladia types. It is a gelling agent used in the preparation of culture media and other bacteriological applications.
The main advantage of this agar is the absence of inhibitors, which could interfere in microorganisms´ growth. It has excellent transparency, high hysteresis and very reliable reproducibility. Each batch produced is thoroughly tested for biological performance against a battery of known bacterial cultures in order to ensure proper growth characteristics and absence of inhibitors.

CAS number: 9002-18-0

Specifications

• Appearance: White cream powder
• Moisture: Less than 10%
• Ashes: ≤ 4.5%
• Gel strength (1.5%, Nikan): 800 –1100 g/cm2
• pH (1.5%) before autoclaving: 7.0 ± 0.4
• pH (1.5%) after autoclaving: 6.5 ± 0.4
• Melting point (1.5%): 85 ± 5 °C
• Gelling point (1.5%): 35 ± 3 °C
• Transparency (1.5%): ≤ 12 NTU
• Colorimetry (absorbance) 450 nm: ≤ 0.200
• Particle size: 95% Over sieve 60

Rate of use: It is used in concentrations from 1.2% to 1.6%

Microbial test:
– Standard plate count: Less than 3000 cfu/g
– Yeasts and molds: Less than 100 cfu/g
– Coliforms: Less than 3 cfu/g
– E.coli: Negative
– Salmonella: Negative

Soluble in: water

Storage: Store at room temperature. Once opened, keep powdered medium closed to avoid hydration.
The dehydrated Agar should be homogeneous, free flowing and beige in color.