Human Telomere Length Quantification qPCR Assay Kit (Relative ）

The Telomere Length Quantification qPCR Assay Kit (Relative) for human: A SYBR Green PCR SuperMix can perform specific and sensitive detection in a wide range, and is suitable for standard and rapid thermal cyclers. The SYBR Green I dye in the master mix can analyze multiple target nucleic acids without the need to synthesize sequence-specific probes. Antibody method hot-start Taq enzyme can effectively inhibit the amplification caused by primer non-specific annealing

Advantages

• Get results quickly, saving 50% of time.
• Optimized ready-to-use master mix for rapid PCR reactions.
• Accurate detection of various starting amounts of templates,stable amplification,and highly reproducible quantitative results.
• Balanced ration of K+ and NH4+ions and independent ROX reference Dye packaging, suitable for all real-time PCR instruments.

Introduction
Telomere (Telomere) is a DNA sequence at the end of a eukaryotic cell chromosome composed of multiple repeating nucleotide elements (TTAGGG) in tandem. In addition to providing a buffer for non-transcribed DNA, it can also protect the end of the chromosome from fusion and Degenerate, protect chromosome structure stability and genetic integrity.

Telomere is the most important and accurate indicator of a person's aging rate. Its initial length is determined by genetic and environmental factors, and will decrease over time. Studies have shown that telomere length is closely related to DNA repair, aging, apoptosis, and tumorigenesis.

Therefore, accurate and repeatable measurement of telomere length is particularly important for researchers. This product mainly uses relative quantitative qPCR to directly compare the average telomere length of the sample, that is, the copy number ratio (T/S) of the telomere repeat sequence (Tel) and the genome single copy gene (SCR) copy number (T/S) is used as the telomere relative length. Single-copy gene primers (SCR) specifically recognize and amplify the 78 bp long region on human chromosome 11.

The primer set in the kit has passed the test to ensure:
- efficient and reliable quantification;
- no non-specific amplification.

Each set of primers has been verified by amplification curve efficiency (E>98%, R2>0.99), melting curve analysis and gel electrophoresis verification.

Components of Human Telomere length Kit for 100 rcs

• 2X qPCR Mastermix: 1000 µl
• Telomere Primer Mix:    50 µl
• Single Copy Reference Primer: 50 µl
• 50X Rox: 250µl
• RNase-free ddH2O: 1 ml

Principle Telomere Kit length Kit
The Human Telomere Length Quantification qPCR Assay Kit SYBR Green PCR SuperMix (relative) can perform specific and sensitive detection in a wide range, and is suitable for standard and rapid thermal cyclers. The SYBR Green I dye in the master mix can analyze multiple target nucleic acids without the need to synthesize sequence-specific probes.

Antibody method hot-start Taq enzyme can effectively inhibit the amplification caused by primer non-specific annealing. At the same time, the PCR formula is optimized, suitable for the amplification of low-concentration templates, so that quantitative PCR can obtain a good standard curve in a wide quantitative area.

Kit application
This product is designed to confirm the average length of chromosomal telomeres in a sample by the ratio (T/S) of the copy number of telomere gene (Tel) and the copy number of single copy gene (SCR). It is for research use only and is not approved for clinical or in vitro diagnosis.

Please note: 0.5ng to 5ng of genomic DNA can be used in a 20 µl system.