Phoretix1D – Automatic SDS PAGE and Western Blot Analysis Software

Phoretix 1D is the complete solution for analysing gel electrophoresis and Immunoblot images, it offers:
• Speed. Automate your analysis and get results in less than a minute per image.
• Objectivity. Unmatched automation reduces the need for manual intervention.
• Reproducibility. Consistent analysis results between different users, labs and different imaging devices.
• Quantitation. Enables reliable, accurate quantitation of gel and blot images.

Automatic detection and quantification of gel electrophoresis data

Phoretix 1D offers automatic lane and band detection or, for even deeper automation and standardisation of your workflow, user-defined protocols which allow full one-click analysis of gel or Western blot images. This removes the potential for operator error entirely.

Coupled with intuitive background removal using industry-leading algorithms, molecular weight calculation, quantity calibration and normalisation Phoretix 1D has all the modern tools required for comprehensive experimental analysis.

Harmonise your lab equipment

Our software is device agnostic, so can help reduce costs as it can be used with your existing laboratory equipment, it also works with all industry-standard image formats: .TIF/.TIFF/.GEL

Phoretix 1D supports 8-bit, 16-bit (recommended) and higher uncompressed TIFF images. Multi-page TIFFs are supported and each page is interpreted as a “channel” (all of which must have the same dimensions and pixel format). .JPG/.JPEG/.PNG/.BMP
While not recommended because of their low bit-depth and (sometimes) compression artifacts, these image formats can be still be analysed.

Compatibility

Phoretix 1D is compatible with the latest versions of both Windows (currently Windows 11) and macOS operating systems (up to and including Ventura).

Manual