pcDNA3-vector, td-EosFP, FLAG®-tagged, lyophilized DNA

nr kat.: VS-FLP10030

Opakowanie: 10 ug

Cena brutto: do wyceny

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nr kat.: VS-FLP10030

Opis

Two variants of EosFP are available from MoBiTec: The tetrameric wildtype protein (EosFP) and a pseudomonomeric variant in which two copies of an engineered EosFP variant are fused to form a tandem dimer (ptd-EosFP). Both variants express functionally in a wide range of pro- and eukaryotic cells at a temperatures of 37 °C or below. For the labeling of cells or tissues, tetrameric EosFP is the construct of choice. For labeling of subcellular compartements using short oligopeptide signals attached to the marker, both EosFP and ptd-EosFP can be considered. Although some fusion proteins with tetrameric EosFP are possible, the pseudomonomeric variant ptd-EosFP is the recommended construct for protein labeling. Fusions to the N-terminus of ptd-EosFP usually work well. Fusions to the C-terminus are also possible, however, some fusion might fail with proteins requiring a strictly monomeric marker, for instance tubulin.