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SMARTchoice Promoter Selection Plate

nr kat.: SP-001000-01
Cena brutto: do wyceny
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Producent: Horizon Discovery Group
nr kat.: SP-001000-01

Opis

Identify active promoters in adherent cells using the SMARTchoice shRNA Promoter Selection Plate
Make informed decisions in the design of RNA interference experiments using the SMARTchoice Promoter Selection Plate. Plan practical shRNA and shMIMIC microRNA experiments using the optimal promoter choice for your specific cells. In a single matrixed experiment, the SMARTchoice Promoter Selection Plate enables the evaluation of seven different promoters at a range of multiplicities of infection (MOIs) in your cells of interest.

Determine the most active promoter in the specific cells of interest to increase small RNA expression, then order SMARTchoice lentiviral shRNAs or shMIMIC microRNAs with your choice of promoter and reporter.

Product Details

  • SMARTvector 2.0 Non-targeting shRNA Controls (NTCs) with seven well-characterized cellular promoters driving the expression of TurboGFP (Evrogen, Moscow, Russia) are packaged into lentiviral particles and concentrated.
  • High-titer non-targeting control lentiviral particles are arrayed in a convenient 96- well format (see Figure 1) in duplicate serial dilutions so multiple MOIs can be tested.


Highlights

  •     Enables straightforward qualitative assessment of promoters that actively drive expression
  •     Quickly evaluate TurboGFP intensity using a fluorescent microscope, a high content imager, or a microplate reader
  •     Improve shRNA and microRNA expression, resulting in

        - increased performance of shRNA-based silencing
        - increased potency of shMIMIC microRNA gene target down-regulation

 

VIDEO

 

SMARTchoice promoter and reporter options are now available for both shRNA and microRNA vector expression. Explore SMARTchoice Lentiviral shRNA and SMARTchoice Lentiviral shMIMIC microRNAs.

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