Description:
Enzymatic method for the determination of D-Fructose and D-Glucose (total sugars). Based on the spectrophotometric measurement of NADPH produced through the reactions, after addition of hexokinase (HK), phosphoglucose isomerase (PGI) and Glucose-6-phosphate dehydrogenase (G6PDH).
Features:
– Rapid reactions
– Prevention of tanins inhibition (PVPP included)
– Suitable for manual and micro volume formats
Applications:
Wine, beer, fruit juices, milk, dietetic foods, bread, jam, honey, ice-creams, fruit and vegetables, pharmaceuticals, cosmetics and biological samples.
See applications for Wine, Food, Feed, Dairy, Fermentation and Biofuel industries.
Specifications:
– Quantity: 110 tests (manual)
– Reaction volume: 2.34 mL
– Range: 2-800 mg/L
– Detection limit: 0.66 mg/L
Components:
– Solution 1 (assay buffer)
– Solution 2 (NADP++ ATP + PVP)
– Suspension 3 (enzymes HK+G6PDH)
– Suspension 4 (enzyme PGI)
– Solution 5 (D-Fructose/D-glucose standard solution- 0.20 mg/mL of each sugar).