1-Step™ Ultra TMB-Blotting Solution

Description

This precipitating, colorimetric Western blot substrate for HRP provides high sensitivity, increased signal-to-noise ratio and low background compared to many other chromogenic substrates. The blotting solution contains soluble TMB (3,3«,5,5«-tetramethylbenzidine), which reacts very quickly with horseradish peroxidase enzyme to produce an insoluble dark blue precipitate that does not fade or flake. The substrate is compatible with both nitrocellulose and PVDF membranes. The blotting solution is supplied ready to use with no mixing required.

Highlights:

• Fast - protein bands visible in less than one minute

• Fade-resistant - protein bands stable after membrane drying

• Sensitive - detection limit similar to Pierce ECL Western Blotting Substrate

• Chromogenic - no special equipment needed for visualization; produces dark blue bands

• Ready to use - no organic solvents are required to dissolve; no dilution necessary for use

Recommended for:

• Chromogenic Western blot detection
• Chromogenic immunohistochemistry detection
• Nitrocellulose membrane blots
• PVDF membrane blots

Formulation: Generates an insoluble dark blue precipitate compatible with nitrocellulose and PVDF membranes

Sufficient For: 25 mini blot experiments at 10mL each