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FastGene® PAGE Gel 4-12% -12 well (8x10 cm)

nr kat.: PG-S412
Opakowanie: 10 szt./op.
Cena brutto: 793,47 zł 793.47
Cena netto: 645,10 zł
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Producent: Nippon
nr kat.: PG-S412

Opis

Pouring gels for protein separation is time consuming and prone to error, thus the replacement by precast protein gels is a progress, which facilitates lab work immensly. Additionally FastGene® Precast Protein Gels are due to their proprietary gel casting method more uniform than every self cast gel and thus offer the advantage of  consistency and reproducibility in the results.

FastGene® Precast Protein Gels are available in a variety of homogenous and gradient gels. They are formulated for denaturing as well as native gel electrophoresis – depending on the used running and transfer buffer. Due to their cast in a neutral pH environment the hydrolysis of polyacrylamide is reduced thus an increased gel stability and superior band resolution are the result. Further advantages are an optimised running performance, larger loading volume, extra tall wells in order to avoid a lane-to-lane overflow, a higher transfer efficiency and a shelf life of 12 months.

The gels are compatible with different common protein electrophoresis gel tanks like from Bio-Rad Hoefer and our FastGene® Electrophoresis Unit. In the folder “Compatibility” you will find listed gel tanks for which the compatibility is already tested, if you use another system we offer to send you a sample, thus you can check if your system is compatible.

 

Compatibility:

FAQ


What is the composition of the precast gels? Are they comparable to traditional Tris Glycine or Bis-Tris gels?

The FastGene precast gels are based on Bis-Tris and are 1mm thick.
 
How do the precast gels perform with instable proteins? Are there special recommendations for the loading and buffer to run native gels to stabilize the protein next to the ones described in your manuals?

If the protein is heat sensitive, than the running condition need to be at 4°C. Our gel can only run acid protein und alkaline condition. We usually set the running buffer at pH 8.8, but this is only a suggested pH value, customers need to test and get their satisfied running pH. The running time may be long.
 
Which buffer can I used for these gels?

For FastGene Gels it is necessary to use MOPS buffer. We offer this buffer as dry pellets in pouches- only mixing with water is necessary. This buffer is also included in the samples.
 
Are your gels keratin free?

The gel is produced keratin free, but there are no QC data available.
 
Do you recommend wet- or semi-dry-blotting for the Precast Gels?

We recommend to use the wet transfer, because we have seen better results compared to the semi-dry transfer. We usually add 10 % Methanol in the transfer buffer, for smaller proteins we will increase the Methanol concentration to 20 %.
 
What is the pH of the stacking and separating part of the gel?

The pH is 6.4

Dane techniczne

Opakowanie 10 szt./op.

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